Dung, D.C. and Nghia, H.L. and Veiko, V.P. and Golubok, A.O. and Yakovlev, E.B. (2010) Glass nanopipette: Fabrication and application for studying living cells. In: 3rd International Conference on the Development of Biomedical Engineering in Vietnam, 11 January 2010 through 14 January 2010, Ho Chi Minh City.
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In this paper, we show fabrication of glass nanopipettes by laser heating and pulling. Then the nanopipette is used as the probe of scanning force microscope for studying living cells. We can get topographical image and mechanical properties of cells. Inside the nanopipette we can fulfill the liquid and metal wire for making an electrode. This electrode can be used for measuring ion current of channels through cell membrane by patch-clamp method. © Springer-Verlag 2010.
Item Type: | Conference or Workshop Item (Paper) |
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Divisions: | Faculties > Faculty of Control Engineering Faculties > Faculty of Special Equipments |
Identification Number: | 10.1007/978-3-642-12020-6_31 |
Uncontrolled Keywords: | Biomedical engineering; Electrodes; Electrophysiology; Fabrication; Glass; Ion currents; Living cell; Mechanical properties of cells; Metal wires; Nanopipettes; Patch clamp; Patch clamp method; Topographical images; Biomechanics |
Additional Information: | Conference code: 81635. Language of original document: English. |
URI: | http://eprints.lqdtu.edu.vn/id/eprint/10174 |