Evaluation of Microalgal Bacterial Dynamics in Pig-Farming Biogas Digestate under Impacts of Light Intensity and Nutrient Using Physicochemical Parameters

Determination of the dynamics between microalgae and bacteria in pig farming biogas digestate is vital for a consistent and reliable application towards sustainable wastewater treatment and biofuel production. This study assesses the reliability of using physicochemical parameters as indicators for the rapid evaluation of microalgal bacterial dynamics in real digestate under impacts of light, nutrient loads, and N:P ratios. The relationship between variation profiles of nutrients, biomass and physicochemical properties in each experiment was analyzed. High light and high nutrient load enhanced biomass growth and nutrient removal rate. Ammonium addition (high N:P ratio) elevated NH3 level which inhibited the growth of microalgae, subsequently reducing the biomass growth and nutrient removal. Low N:P ratio triggered the accumulation of phosphorus and the growth of chlorophyll-a but exerted little influence on treatment. Variation profiles of dissolved oxygen, nutrient and biomass were highly consistent in every experiment allowing us to identify the shift from microalgal to bacterial predomination under unfavorable conditions including low light intensity and high N:P ratio. Strong linear correlation was also found between total nitrogen removal and electrical conductivity (R2 = 0.9754). The results show the great potential of rapid evaluation of microalgal bacterial dynamics for large scale system optimization and modelling. © 2022 by the authors.