LE QUY DON
Technical University
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Saturated confocal fluorescence microscopy with linear polarization modulation

Le, V. (2023) Saturated confocal fluorescence microscopy with linear polarization modulation. Optical and Quantum Electronics, 55 (1). ISSN 03068919

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Abstract

In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Item Type: Article
Divisions: Faculties > Faculty of Special Equipments
Identification Number: 10.1007/s11082-022-04386-0
Uncontrolled Keywords: Elastic waves; Fluorescence; Fluorescence microscopy; Optical resolving power; Polarization; Transfer functions, Confocal fluorescence microscopy; Confocal scanning fluorescence microscopy; Gaussians; Higher-frequency components; Linear polarization; Plane wave illumination; Polarization-modulation; RL algorithm; Superresolution, Wave propagation
URI: http://eprints.lqdtu.edu.vn/id/eprint/10636

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