Super-resolution for confocal scanning fluorescence microscopy by rotating line-shape point spread function of super-oscillation technique

In this paper, we introduce the super-oscillation technique with the binary phase mask to obtain the line-shape point spread function which produces a super-resolution image in confocal scanning fluorescence microscopy. By rotating the binary phase mask for the four different angles, the line-shape point spread function spins the corresponding angles. From obtained dataset, the Richardson–Lucy deconvolution algorithm is used to acquire the super-resolution image. The imaging results demonstrated the ability of the proposed method to improve the spatial resolution in the confocal scanning fluorescence microscopy. Graphical abstract: Figure not available: see fulltext. © 2023, The Author(s), under exclusive licence to EDP Sciences, SIF and Springer-Verlag GmbH Germany, part of Springer Nature.