Le, V. and Pham, M. and Hoang, V. (2020) Axial Resolution Enhacement of Light-Sheet Microscopy via two Light-Sheets. In: 16th IEEE Asia Pacific Conference on Circuits and Systems, APCCAS 2020, 8 December 2020 through 10 December 2020.
Axial Resolution Enhacement of Light-Sheet Microscopy via two Light-Sheets..pdf
Download (387kB) | Preview
Abstract
Light-sheet fluorescence microscopy has many advantages including high-speed, noninvasive and low photobleaching and photodamage. The light-sheet thickness of light-sheet microscopy is used to determine the axial resolution. However, the light-sheet thickness is limited by the light diffraction. In order to beyond this limit, inhere, we introduce a novel way based on the use of two light-sheets to achieve the enhancement of the axial resolution of light-sheet microscopy. Two images are captured by using both Gaussian light-sheet and negative light-sheet beams. From these two images, a new relationship between them is built to achieve the axial resolution image higher than the image of Gaussian light-sheet. Experimental result is performed, indicating that the effectiveness of the proposed method is better than traditional light-sheet microscopy. © 2020 IEEE.
Item Type: | Conference or Workshop Item (Paper) |
---|---|
Divisions: | Faculties > Faculty of Radio-Electronic Engineering Faculties > Faculty of Special Equipments |
Identification Number: | 10.1109/APCCAS50809.2020.9301664 |
Uncontrolled Keywords: | Fluorescence microscopy; Photobleaching; Axial resolutions; Gaussians; High Speed; Light diffraction; Light sheet; Light-sheet microscopies; Photo-damage; Gaussian beams |
Additional Information: | Conference code: 166223. Language of original document: English. |
URI: | http://eprints.lqdtu.edu.vn/id/eprint/8842 |