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Axial Resolution Enhacement of Light-Sheet Microscopy via two Light-Sheets

Le, V. and Pham, M. and Hoang, V. (2020) Axial Resolution Enhacement of Light-Sheet Microscopy via two Light-Sheets. In: 16th IEEE Asia Pacific Conference on Circuits and Systems, APCCAS 2020, 8 December 2020 through 10 December 2020.

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Abstract

Light-sheet fluorescence microscopy has many advantages including high-speed, noninvasive and low photobleaching and photodamage. The light-sheet thickness of light-sheet microscopy is used to determine the axial resolution. However, the light-sheet thickness is limited by the light diffraction. In order to beyond this limit, inhere, we introduce a novel way based on the use of two light-sheets to achieve the enhancement of the axial resolution of light-sheet microscopy. Two images are captured by using both Gaussian light-sheet and negative light-sheet beams. From these two images, a new relationship between them is built to achieve the axial resolution image higher than the image of Gaussian light-sheet. Experimental result is performed, indicating that the effectiveness of the proposed method is better than traditional light-sheet microscopy. © 2020 IEEE.

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculties > Faculty of Radio-Electronic Engineering
Faculties > Faculty of Special Equipments
Identification Number: 10.1109/APCCAS50809.2020.9301664
Uncontrolled Keywords: Fluorescence microscopy; Photobleaching; Axial resolutions; Gaussians; High Speed; Light diffraction; Light sheet; Light-sheet microscopies; Photo-damage; Gaussian beams
Additional Information: Conference code: 166223. Language of original document: English.
URI: http://eprints.lqdtu.edu.vn/id/eprint/8842

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