Hoang, X. and Le, V. and Pham, M.N. (2020) Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions. In: 6th EAI International Conference on Industrial Networks and Intelligent Systems, INISCOM 2020, 24 August 2020 through 28 August 2020.
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In this paper, we propose a new method to obtain the improvement of lateral axial resolution of confocal fluorescence microscopy. In this method, we employ two different beams to illuminate the sample: (1) the Gaussian beam; (2) the donut beam. Two different images are produced from these two illumination beams. A higher resolution image is generated by a multi-relationship between these two image. A set of simulation and experimental results are employed to compare the effectiveness of proposed method with the traditional confocal fluorescence microscopy. These results demonstrated that our method can be employed to achieve the resolution-enhancement of confocal fluorescence microscopy. © 2020, ICST Institute for Computer Sciences, Social Informatics and Telecommunications Engineering.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | Faculties > Faculty of Radio-Electronic Engineering Faculties > Faculty of Special Equipments |
| Identification Number: | 10.1007/978-3-030-63083-6_6 |
| Uncontrolled Keywords: | Fluorescence; Fluorescence microscopy; Intelligent systems; Optical transfer function; Axial resolutions; Confocal fluorescence microscopy; Higher resolution images; Illumination beams; Resolution enhancement; Resolution improvement; Gaussian beams |
| Additional Information: | Conference code: 252119. Language of original document: English. |
| URI: | http://eprints.lqdtu.edu.vn/id/eprint/9105 |
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