LE QUY DON
Technical University
VietnameseClear Cookie - decide language by browser settings

An impedance biosensor for monitoring cancer cell attachment, spreading and drug-induced apoptosis

Anh-Nguyen, T. and Tiberius, B. and Pliquett, U. and Urban, G.A. (2016) An impedance biosensor for monitoring cancer cell attachment, spreading and drug-induced apoptosis. Sensors and Actuators, A: Physical, 241. pp. 231-237. ISSN 9244247

Text
Manuscript _Submit.pdf

Download (974kB) | Preview

Abstract

In the last three decades, Electrical Cell-substrate Impedance Sensing (ECIS) has emerged as a powerful technique for in vitro cellular research and preclinical testing. In this study, a cell-based impedance biosensor based on ECIS technique, which integrates microelectrode arrays with a small culture chamber has been developed for monitoring the attachment, spreading and drug-induced apoptosis of very few MCF-7 breast cancer cells. It was found out that cell spreading caused a significant increase of impedance magnitude in the frequency range between 10 kHz and 100 kHz and also pronounced phase shift. Using an electrical equivalent circuit to model the measured spectra, the morphological change of cells was analyzed. Cell spreading induced a slight decrease of membrane capacitance of 2% and a prominent increase of membrane resistance of 57% within incubating for nearly one day. For pharmaceutical assessment, the cells were treated with different concentrations of an anti-cancer drug (Cisplatin) in the range from 10 μM to 50 μM. We observed a fast decrease of impedance magnitude within the initial 4 h of treatment with concentrations of 25 μM and 50 μM. The results derived from this study demonstrated the usability of cell-based impedance microbiosensors in cell biology and cancer research. © 2016 Elsevier B.V.

Item Type: Article
Divisions: Faculties > Faculty of Physical and Chemical Engineering
Identification Number: 10.1016/j.sna.2016.02.035
Uncontrolled Keywords: Biosensors; Cell death; Cytology; Diseases; Electrodes; Microelectrodes; Phase shift; Phase shifters; Platinum compounds; Anticancer drug; Cancer cell attachment; Cancer cells; Cell-based biosensor; Electrical equivalent circuit; Impedance measurement; MCF-7 breast cancer cells; Membrane capacitances; Cells
Additional Information: Language of original document: English.
URI: http://eprints.lqdtu.edu.vn/id/eprint/9837

Actions (login required)

View Item
View Item